These features, combined with their impressive regenerative capacity, also make zebrafish ideal for studying vertebrate tissue and organ regeneration. Moreover, their optical transparency and readily available suite of genetic tools (Tol1 and 2 tranposase-mediated transgenesis, Gal4/UAS gene expression system, Cre/loxP genetics, etc.) and fluorescent reporters (e.g., gCaMP, lifeact fluorescent proteins, etc.) have made zebrafish the premier model system for studying vertebrate biology in real time. The fecundity, rapid development, external fertilization, amenability to both forward and reverse genetic approaches, conservation of core vertebrate protein-coding genes, small size, and inexpensive husbandry costs make zebrafish a powerful vertebrate model for studying human physiology and disease.
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